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Comparison of the milk-derived EV proteome from this study to the EV database Vesiclepedia. A, All protein entries from Vesiclepedia were downloaded and imported into FunRich for overlay analysis with the milk-derived EV proteome from this study, which is shown in the Venn diagram. B, Western blot analysis was performed <t>for</t> <t>OLAH</t> (∼30 kDa) and <t>PTHLH</t> (∼20 kDa and ∼24 kDa) on milk-derived EV from donors 4, 5, 6, and 7.
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Comparison of the milk-derived EV proteome from this study to the EV database Vesiclepedia. A, All protein entries from Vesiclepedia were downloaded and imported into FunRich for overlay analysis with the milk-derived EV proteome from this study, which is shown in the Venn diagram. B, Western blot analysis was performed <t>for</t> <t>OLAH</t> (∼30 kDa) and <t>PTHLH</t> (∼20 kDa and ∼24 kDa) on milk-derived EV from donors 4, 5, 6, and 7.
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Comparison of the milk-derived EV proteome from this study to the EV database Vesiclepedia. A, All protein entries from Vesiclepedia were downloaded and imported into FunRich for overlay analysis with the milk-derived EV proteome from this study, which is shown in the Venn diagram. B, Western blot analysis was performed for OLAH (∼30 kDa) and PTHLH (∼20 kDa and ∼24 kDa) on milk-derived EV from donors 4, 5, 6, and 7.

Journal: Molecular & Cellular Proteomics : MCP

Article Title: Comprehensive Proteomic Analysis of Human Milk-derived Extracellular Vesicles Unveils a Novel Functional Proteome Distinct from Other Milk Components *

doi: 10.1074/mcp.M116.060426

Figure Lengend Snippet: Comparison of the milk-derived EV proteome from this study to the EV database Vesiclepedia. A, All protein entries from Vesiclepedia were downloaded and imported into FunRich for overlay analysis with the milk-derived EV proteome from this study, which is shown in the Venn diagram. B, Western blot analysis was performed for OLAH (∼30 kDa) and PTHLH (∼20 kDa and ∼24 kDa) on milk-derived EV from donors 4, 5, 6, and 7.

Article Snippet: Proteins of interest were detected by immunoblotting using mouse anti-human flotillin-1 (clone 18, BD Biosciences, San Jose, CA), mouse anti-human CD9 (HI9a, BioLegend, Fell, Germany), rabbit anti-human OLAH (HPA037948, Atlas Antibodies, Stockholm, Sweden), rabbit anti-human PTHLH (PA5–40799, Thermo Fisher, Rockford, IL), mouse anti-human MPZL1 (SAB1406763, Sigma), mouse anti-human EHD3 (ab194512, Abcam, Cambridge, UK).

Techniques: Derivative Assay, Western Blot